Granzyme A (E9L8S) Rabbit mAb #96715

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	28
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Granzyme A (E9L8S) Rabbit mAb recognizes endogenous levels of total Granzyme A protein. This antibody does not cross-react with human Granzyme B, H, K, or M proteins.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val242 of human Granzyme A protein.

Background

Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).

Database Links

UniProt ID: P12544

Entrez-Gene Id: 3001

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

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