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Render Timestamp: 2024-11-22T11:06:41.648Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:29:52.126
Product last modified at: 2024-06-27T13:37:09.301Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. Granzyme F Antibody

Granzyme F Antibody #16433

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 30-40
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Granzyme F Antibody recognizes endogenous levels of total Granzyme F protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln197 of mouse Granzyme F protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). In mice, ten family members have been identified: A, B, C, D, E, F, G, K, M, and N (2). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein (3). Granzyme F induces a novel cell death pathway consisting of externalization of phosphatidylserine (PS), nuclear condensation, mitochondrial damage, cytochrome c release, single-stranded DNA nicking, and caspase inactivation (4). CTLs from mice with reduced expression of Granzyme F and C display diminished cytotoxicity (5).  

    Database Links

    UniProt ID: P08883


    Entrez-Gene Id: 14943


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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