Granzyme H (E3H7W) Rabbit mAb #99695
- WB
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25-35 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Granzyme H has chymotrypsin-like thioester activity with a preference for hydrophobic, aromatic amino acid residues, such as phenylalanine, tyrosine, or methionine, at the P1 site (4,5). Granzyme H is predominantly expressed at high levels in NK cells, but not in T lymphocytes, and has also been described in mast cells (6-8). After perforin-mediated entry into a target cell, Granzyme H induces many hallmarks of programmed cell death, such as mitochondrial depolarization, generation of reactive oxygen species, DNA degradation, and chromatin condensation (9,10).
- Trapani, J.A. (2001) Genome Biol. 2, REVIEWS 3014.
- Lord, S.J. et al. (2003) Immunol. Rev. 193, 31-8.
- Trapani, J.A. and Sutton, V.R. (2003) Curr. Opin. Immunol. 15, 533-43.
- Edwards, K.M. et al. (1999) J Biol Chem 274, 30468-73.
- Mahrus, S. and Craik, C.S. (2005) Chem Biol 12, 567-77.
- Sedelies, K.A. et al. (2004) J Biol Chem 279, 26581-7.
- Bade, B. et al. (2005) Int Immunol 17, 1419-28.
- Rönnberg, E. et al. (2014) Int Arch Allergy Immunol 165, 68-74.
- Fellows, E. et al. (2007) Blood 110, 544-52.
- Hou, Q. et al. (2008) Mol Immunol 45, 1044-55.
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