Granzyme K (E6Z6O) Rabbit mAb #98946
- WB
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17-27 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Granzyme K has tryptase-like activity with a preference for cleaving its specific substrates after basic residues, arginine or lysine (4-7). Granzyme K is expressed by NK T cells, cytotoxic T cells, and NK cells (8,9). After perforin-mediated entry into a target cell, Granzyme K induces apoptosis via generation of reactive oxygen species, DNA damage, and BID-dependent mitochondrial damage (10-12).
- Trapani, J.A. (2001) Genome Biol. 2, REVIEWS 3014.
- Lord, S.J. et al. (2003) Immunol. Rev. 193, 31-8.
- Trapani, J.A. and Sutton, V.R. (2003) Curr. Opin. Immunol. 15, 533-43.
- Wilharm, E. et al. (1999) J Biol Chem 274, 27331-7.
- Mahrus, S. and Craik, C.S. (2005) Chem Biol 12, 567-77.
- Carter, C.R. et al. (1996) Cell Immunol 172, 235-45.
- Bovenschen, N. et al. (2009) J Biol Chem 284, 3504-12.
- Bratke, K. et al. (2008) Thorax 63, 1006-11.
- Bade, B. et al. (2005) Int Immunol 17, 1419-28.
- Zhao, T. et al. (2007) J Biol Chem 282, 12104-11.
- Zhao, T. et al. (2007) Cell Death Differ 14, 489-99.
- MacDonald, G. et al. (1999) J Exp Med 189, 131-44.
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