Render Target: STATIC
Render Timestamp: 2024-11-21T13:26:32.763Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:23:43.978
Product last modified at: 2024-05-30T07:01:20.239Z
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PDP - Template Name: Polyclonal Antibody (Epitope Tag)
PDP - Template ID: *******04464df

GSK-3β-Tag Antibody #9325

Filter:
  • WB

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GSK-3β-Tag Antibody is optimized to detect transfected levels of GSK-3β-tagged proteins and weakly detects endogenous GSK-3β (46 kDa).

    Species Reactivity:

    All Species Expected

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic GSK-3β-Tag peptide. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    Short phosphorylatable peptide tags, such as the GSK-3β-tag, can be used to determine translocation of effector proteins in type III secretion systems (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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