HDGF (E3P7K) Rabbit mAb #42105
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40-45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
HDGF (E3P7K) Rabbit mAb recognizes endogenous levels of total HDGF protein. Based on the amino acid sequence of the antigenic peptide, this antibody is expected to recognize isoforms 1-3 of HDGF (HDGF-A, -B and -C).
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala229 of human HDGF protein.
Background
HDGF (hepatoma-derived growth factor) acts as both a transcription factor and a secreted protein to regulate multiple cellular functions such as transformation, apoptosis, angiogenesis, proliferation, and metastasis. HDGF is involved in the progression of multiple human cancers, and its elevated expression is a negative prognostic marker in cancer progression (1,2).
Limited Uses
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