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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

HELLS Antibody #7998

Filter:
  • WB
Western Blotting Image 1: HELLS Antibody
Western blot analysis of extracts from various cell lines using HELLS Antibody.

To Purchase # 7998

Cat. # Size Qty. Price Ships
7998S 100 µl
$306

Supporting Data

REACTIVITY H M Mk
SENSITIVITY Endogenous
MW (kDa) 97
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HELLS Antibody recognizes endogenous levels of total HELLS protein. HELLS Antibody is predicted to detect multiple isoforms of this protein.

Species Reactivity:

Human, Mouse, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys520 of human HELLS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

HELLS, or LSH1, is a lymphoid-specific helicase thought to be involved in cellular proliferation and leukemogenesis (1,2). It is believed to be a chromatin remodeler and is required for DNMT1-mediated methylation maintenance and DNMT3A/DNMT3B-mediated de novo methylation. The role of HELLS in methylation maintenance was thought to be largely confined to repetitive DNA sequences, including major and minor satellite sequences, rather than single copy genes (3,4); recent evidence suggests a role in genome-wide cysteine methylation at non-repeat sequences (5). De novo methylation maintenance is associated with silencing of specific genes, some known to be involved in pluripotency and lineage commitment (6,7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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