HELQ (D4K2O) Rabbit mAb #19436

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	130
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HELQ (D4K2O) Rabbit mAb recognizes endogenous levels of total HELQ protein. In some cell lines, this antibody cross-reacts with a 200 kDa band of unknown identity by western blot.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1091 of human HELQ protein.

Background

DNA interstrand crosslinks (ICLs) are toxic DNA lesions caused by environmental agents as well as some chemotherapeutic drugs. These lesions are repaired via multiple DNA repair pathways. HELQ (also known as HEL308) is a 3’-5’ DNA helicase that colocalizes with stalled DNA replication forks in response to DNA damage, and contributes to the repair of ICL lesions through ATR signaling (1,2). HELQ interacts with various DNA repair proteins, including FANCD2, RAD51, RAD51 paralogs, and ATR (1-3). HELQ-deficient mice exhibit reduced fertility as well as interstrand crosslink (ICL) repair defects, and are prone to tumors (3).

Database Links

UniProt ID: Q8TDG4

Entrez-Gene Id: 113510

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For Research Use Only. Not For Use In Diagnostic Procedures.

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