HER2 (Trastuzumab Biosimilar) Human mAb #10583

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Human IgG1 kappa

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

HER2 (Trastuzumab Biosimilar) Human mAb is a biosimilar antibody for Trastuzumab. Trastuzumab is a therapeutic human monoclonal antibody directed against human HER2 used to treat HER2-positive breast cancer.

Endotoxin

<0.1 EU/μg of antibody

Product Usage Information

This product is intended for research use only (RUO). Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

HER2 (Trastuzumab Biosimilar) Human mAb was confirmed to bind to its intended target protein HER2 using flow cytometry.

Species Reactivity:

Human

Source / Purification

HER2 (Trastuzumab Biosimilar) Human mAb is produced at Cell Signaling Technology. Trastuzumab is an antibody directed against the extracellular region of human HER2.

Background

The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).

Database Links

UniProt ID: P04626

Entrez-Gene Id: 2064

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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