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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

HER4/ErbB4 (111B2) Rabbit mAb #4795

Filter:
  • WB
  • IP
Western Blotting Image 1: HER4/ErbB4 (111B2) Rabbit mAb
Western blot analysis of extracts from various cell lines, using HER4/ErbB4 (111B2) Rabbit mAb .

To Purchase # 4795

Cat. # Size Qty. Price Ships
4795T 20 µl
$143 Jan 22
4795S 100 µl
$336 Jan 22

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 180
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #72663.

Protocol

Specificity / Sensitivity

HER4/ErbB4 (111B2) Rabbit mAb detects endogenous levels of ErbB4 protein. This antibody does not cross-react with other ErbB family members.

Species Reactivity:

Human, Mouse

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human ErbB4.

Background

Research studies have implicated the HER/ErbB receptor tyrosine kinase family in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in research studies suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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