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Product last modified at: 2025-01-03T08:00:57.061Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Hexokinase II (F9Q4T) Rabbit mAb #55205

Filter:
  • WB
  • IP
  • F
Western Blotting Image 1: Hexokinase II (F9Q4T) Rabbit mAb
Western blot analysis of extracts from various cell lines using Hexokinase II (F9Q4T) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of hexokinase II protein in A549 and MKN74 cells is consistent with the predicted expression pattern.

To Purchase # 55205

Cat. # Size Qty. Price Ships
55205S 100 µl
$370

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 102
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:50
Flow Cytometry (Fixed/Permeabilized) 1:600 - 1:2400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Hexokinase II (F9Q4T) Rabbit mAb recognizes endogenous levels of total hexokinase II protein. This antibody does not cross-react with hexokinase I protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human hexokinase II protein.

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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