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Product last modified at: 2025-01-01T09:03:45.213Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HGK (D19H10) Rabbit mAb #5146

Filter:
  • WB
Western Blotting Image 1: HGK (D19H10) Rabbit mAb
Western blot analsysis of extracts from various cell lines using HGK (D19H10) Rabbit mAb.

To Purchase # 5146

Cat. # Size Qty. Price
5146T 20 µl
$153
5146S 100 µl
$339

Supporting Data

REACTIVITY H M Mk
SENSITIVITY Endogenous
MW (kDa) 150
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HGK (D19H10) Rabbit mAb detects endogenous levels of total HGK protein.

Species Reactivity:

Human, Mouse, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala553 of human HGK protein.

Background

HGK (MAP4K4 or mitogen-activated protein kinase kinase kinase kinase 4) is a serine/threonine kinase that belongs to the mammalian STE20/MAP4K kinase family involved in response to environmental stress and cytokines such as TNF-α (1-3). HGK specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway and increases AP-1-mediated transcriptional activity in vivo (1). HGK is broadly expressed in many types of human cancer and cancer cell lines and plays an important role in cell transformation, invasiveness, adhesion and migration (4,5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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