HIF-2α (E7K6E) Rabbit mAb #87179

Filter:

WB
ChIP

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	120
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
ChIP-Chromatin Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

For optimal ChIP and ChIP-Seq results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10⁶ cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Chromatin IP	1:100
Chromatin IP-seq	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HIF-2α (E7K6E) Rabbit mAb recognizes endogenous levels of total HIF-2α protein. This antibody does not cross-react with HIF-1α protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu645 of human HIF-2α protein.

Background

Hypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia (1,2). Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin/proteasome pathway (1,2). Hypoxia inhibits degradation of the α subunit, which leads to its stabilization (1,2). HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions (1,2). There are several isoforms of the HIF α subunit (2). Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers (2). HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity (2). Therefore, HIF-2α likely plays an important role in tumorigenesis (2,3).

Database Links

UniProt ID: Q99814

Entrez-Gene Id: 2034

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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