Render Target: STATIC
Render Timestamp: 2024-12-26T11:04:14.101Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:42.129
Product last modified at: 2024-12-17T19:03:57.541Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HIP1 (E9J9B) Rabbit mAb #90830

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 116
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HIP1 (E9J9B) Rabbit mAb recognizes endogenous levels of total HIP1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser166 of human HIP1 protein.

    Background

    Huntingtin-interacting protein 1 (HIP1), a ubiquitously expressed cytosolic protein, was originally discovered by yeast two-hybrid screening for proteins that interact with the huntingtin protein. HIP1 binds the amino terminus of the huntingtin protein. This interaction is weakened by huntingtin protein mutations that cause expanded polyglutamine repeats, suggesting that HIP1 may play a role in Huntington’s disease (HD) pathogenesis. HIP1, along with its human ortholog, HIP1-related protein (HIP1R) and its yeast ortholog Sla2p share a conserved domain structure; an epsin amino-terminal homology (ENTH) domain, a leucine zipper motif, and a carboxy-terminal domain homologous to TALIN (1,2). The ENTH domain of HIP1 binds clathrin and the adaptor protein AP-2, while the TALIN domain has been shown to bind actin. These interactions suggest that HIP1 plays a role in clathrin-mediated endocytosis (3-6). HIP1 has also been shown to bind receptor tyrosine kinases (RTKs), including the epidermal growth factor receptor (EGFR). Elevated expression levels of HIP1 correlate with elevated levels of EGFR and have been linked to the development and progression of various cancers (7-10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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