HIP2 (D27C4) Rabbit mAb #8226
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Huntingtin-interacting protein-2 (HIP2), also known as E2-25K, is a member of the E2 protein family that is highly expressed in the brain and catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin (2). E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-β, huntingtin, and MHC-heavy chain proteins (3-6). Recent studies have also implicated HIP2 in the control of apoptosis and cell-cycle progression through its ability to regulate the stability of Smac/Diablo and cyclin B1 (7,8).
- Hershko, A. (1988) J Biol Chem 263, 15237-40.
- Chen, Z. and Pickart, C.M. (1990) J Biol Chem 265, 21835-42.
- Song, S. et al. (2003) Mol Cell 12, 553-63.
- de Pril, R. et al. (2007) Mol Cell Neurosci 34, 10-9.
- Flierman, D. et al. (2006) Proc Natl Acad Sci USA 103, 11589-94.
- Kalchman, M.A. et al. (1996) J Biol Chem 271, 19385-94.
- Bae, Y. et al. (2010) Biochem Biophys Res Commun 397, 718-23.
- Bae, Y. et al. (2010) FEBS Lett 584, 4505-10.
Limited Uses
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