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Product last modified at: 2024-09-06T13:45:07.933Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Hippo Pathway: Upstream Signaling Antibody Sampler Kit

Hippo Pathway: Upstream Signaling Antibody Sampler Kit #56612

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Hippo Pathway Proteins Antibody Sampler Kit provides an economical means of detecting proteins that have been identified as upstream regulators of the Hippo Signaling Pathway. The kit provides enough antibody to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Hippo Pathway Proteins Antibody Sampler Kit detects endogenous levels of its target protein.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ala200 of mouse TAZ, Phe608 of human FRMD6, Leu737 of human PTPN14, Gln470 of human merlin, Arg77 of human Ajuba, Pro9 of human MST1, and recombinant protein corresponding to the carboxy terminus of human YAP protein. The sequence region surrounding the YAP epitope is 100% conserved among all known isoforms of YAP.

    Background

    The Hippo Signaling Pathway is an evolutionarily conserved signaling cascade that regulates cell, tissue and organ growth in response to diverse environmental cues, including cell density, mechanical stress, growth factors and cytokines, and metabolic signaling (1,2). In mammals, the core components of this pathway are YAP and TAZ, transcriptional co-activators that regulate the expression of target genes that control cell proliferation and survival (3). The transcriptional control of YAP/TAZ target genes is regulated through a complex kinase cascade involving mammalian sterile-20-like kinases (MST1/2), LATS1/2 kinases and key adaptor proteins. Phosphorylation of YAP and TAZ by LATS kinases results in cytoplasmic sequestration of YAP and TAZ, inhibiting transcriptional activation of their target genes. A number of proteins have been identified that function upstream of MST1/2 and LATS1/2 to influence LATS-mediated phosphorylation of YAP/TAZ. These include the tumor suppressor protein Merlin (4), the FERM-domain containing protein 6 (FRMD6/Willin) (5), the LIM-domain containing adaptor protein Ajuba (6,7), and the protein tyrosine phosphatase PTPN14 (8).

    Database Links

    UniProt ID: Q9GZV5 , P35240 , Q96NE9 , P46937 , Q15678 , Q96IF1 , Q13043


    Entrez-Gene Id: 25937 , 4771 , 122786 , 10413 , 5784 , 84962 , 6789


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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