HLA-E (MEM-E/02) Mouse mAb #73269
- WB
- IHC
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:2000 - 1:8000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1000 - 1:4000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant human HLA-E denatured heavy chain.
Background
HLA-E (HLA class I histocompatibility antigen, alpha chain E) is a non-classical MHC molecule that is expressed by B and T lymphocytes, natural killer (NK) cells, and macrophages, and is overexpressed in some tumors (1-3). Classical HLA class I molecules (HLA-A, -B, and -C) are highly polymorphic, affecting the peptide binding region (PBR). HLA-E has limited polymorphism that is restricted to two functional versions (3,5). HLA-E is a ligand of CD94/NKG2A, -B, and -C receptors, which are expressed on NK cells and T cells and play a role in the innate and adaptive immune system (3-5).
Limited Uses
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