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Product last modified at: 2025-01-10T22:15:10.656Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

HLTF Antibody #43345

Filter:
  • WB
  • IP
Western Blotting Image 1: HLTF Antibody
Western blot analysis of extracts from various cell lines using HLTF Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, Colo 205 cells are low for HLTF expression.

To Purchase # 43345

Cat. # Size Qty. Price Ships
43345S 100 µl
$306

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 130
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HLTF Antibody recognizes endogenous levels of total HLTF protein.

Species Reactivity:

Human, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala998 of human HLTF protein. Antibodies are purified by peptide affinity chromatography.

Background

Helicase-like transcription factor (HLTF), also known as RUSH-1 or SMARCA3, belongs to the SNF2/SWI2 family of ATP-dependent remodelers (1-3). HLTF has been shown to interact with ubiquitin enzyme complexes to promote Lys-63-linked polyubiquitination of PCNA, maintaining genomic stability during DNA damage (4,5). HLTF has double-stranded DNA translocation activity, which can restart replication forks at DNA lesions, providing tumor suppressive functions (6). The HLTF gene is frequently hypermethylated in many different tumor types (7-9).
For Research Use Only. Not For Use In Diagnostic Procedures.
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