hnRNP E1 Antibody #8534
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
hnRNP E1 Antibody recognizes endogenous levels of total hnRNP E1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys237 of human hnRNP E1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
hnRNP E1 is a member of the hnRNP family of proteins that are involved in pre-mRNA processing and mRNA export, localization, stability, and translation (1-6). hnRNP E1 exerts a wide range of biological functions, such as transcriptional activation of mouse MOR gene expression (7), attenuation of alternative splicing of GHR pseudoexon expression (8), stabilization of collagen I and II (9), beta-globin (10), and androgen receptor (11) mRNAs, and regulation of translation of various genes including Dab2, ILEI, and Bag-1 (12,13). hnRNP E1 is ubiquitously expressed. Phosphorylation of hnRNP E1 affects its RNA binding affinity (13,14).
Limited Uses
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