Render Target: STATIC
Render Timestamp: 2024-11-22T11:39:29.819Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:58.638
Product last modified at: 2024-09-30T08:01:50.815Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HOXB7 (E3H4S) Rabbit mAb #65130

Filter:
  • WB
  • IF
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 27
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HOXB7 (E3H4S) Rabbit mAb recognizes endogenous levels of total HOXB7 protein. The antibody also detects a 20 kDa band of unknown origin.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human HOXB7 protein.

    Background

    Homeobox (HOX) proteins are homeodomain transcription factors (1). They bind to sequence-specific DNA motifs (C/G)TAATTG and function as master transcriptional regulators, controlling the coordinated expression of genes involved in embryogenesis and tissue differentiation (1,2). HOXB7 belongs to the B group of HOX proteins and plays a dominant role in facilitating tumor progression in many solid tumors. Overexpression of HOXB7 is found in many types of tumors, including melanoma, ovarian, GI-tract, pancreatic ductal adenocarcinoma (PDAC), lung, gliomas, and breast cancers. Aberrant high expression of HOXB7 is associated with poor prognosis (3). The protein promotes tumor cell proliferation, angiogenesis, epithelial-mesenchymal transition, migration, and invasion, as well as drug resistance through regulation of multiple signaling pathways, such as MAPK, VEGF, FGF, TGF-β, and EGFR/Her2 (4-8). HOXB7 binding to a DNA promoter requires its interaction with a cofactor protein such as PBX2 or CBP for transcriptional activation of downstream targets (9,10). Disruption of the HOXB7/PBX complex formation by antagonist peptide HXR9 can inhibit HOXB7 function and is considered as a potential therapeutic value (11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.