Render Target: STATIC
Render Timestamp: 2024-12-30T11:16:53.217Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:57:28.604
Product last modified at: 2024-12-17T18:57:24.401Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HOXD9 (E3F2T) Rabbit mAb #55962

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HOXD9 (E3F2T) Rabbit mAb recognizes endogenous levels of total HOXD9 protein. This antibody does not cross-react with HOXA9, HOXB9 or HOXC9 proteins. This antibody also weakly recognizes non-specific bands of unknown origin at 55 and 70 kDa in some cell lines.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly159 of human HOXD9 protein.

    Background

    Homeobox protein Hox-D9 (HOXD9) is a sequence-specific transcription factor that is part of a developmental regulatory program that provides cells with specific positional identities on the anterior-posterior axis. HOXD9 is developmentally expressed in structures of either mesodermal or neuro-ectodermal origin, such as developing limbs, gonads, and the central nervous system (1-6). HOXD9 plays a critical role in regulation of limb development, neuronal development, and development of mammary glands and gonads in many organisms (1-6). The HOXD9 gene promoter is found to be hypermethylated and silenced in multiple types of cancer, including breast cancer, melanoma brain metastases, and cholangiocarcinomas (7-9). In addition, HOXD9 expression is increased in other types of cancer, including human glioblastomas and astrocytomas, where expression appears to drive growth of the tumors (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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