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PDP - Template Name: Antibody Duet
PDP - Template ID: *******ad0fa02

PhosphoPlus® HSP27 (Ser82) Antibody Duet #11900

PhosphoPlus® HSP27 (Ser82) Antibody Duet: Image 1
Western blot analysis of extracts from HeLa cells (lane 1) or HSP27 knock-out cells (lane 2) using HSP27 (D6W5V) Rabbit mAb #95357 (upper), and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The absence of signal in the HSP27 knock-out HeLa cells confirms specificity of the antibody for HSP27.

To Purchase # 11900

Cat. # Size Qty. Price Ships
11900S 1 Kit
$651
  • Product Includes
  • Related Products
Product IncludesQuantityReactivityMW(kDa)Isotype
Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb #9709100 µlH M27Rabbit IgG
HSP27 (D6W5V) Rabbit mAb #95357100 µlH27Rabbit IgG

Product Information

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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