HSP47/SERPINH1 (E4A8P) Rabbit mAb #31877

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Supporting Data

REACTIVITY	H Mk
SENSITIVITY	Endogenous
MW (kDa)	46
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:500 - 1:2000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HSP47/SERPINH1 (E4A8P) Rabbit mAb recognizes endogenous levels of total HSP47/SERPINH1 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human HSP47/SERPINH1 protein.

Background

Serpin peptidase inhibitor, clade H, member 1 (serpin H1), also known as heat shock protein 47 (HSP47), is a collagen specific molecular chaperone localized to the endoplasmic reticulum, essential for collagen biosynthesis. Despite its lack of serine proteinase activity, serpin H1 is a member of the serpin superfamily (1,2). Genetic deletion of SERPINH1 or inhibition of its interaction with procollagen has been shown to greatly reduce procollagen secretion from fibroblasts, implicating serpin H1 as a potential target for therapeutic management of fibrosis (3). Moreover, altered serpin H1 expression has been seen in multiple cancer types, including colon carcinoma, head and neck squamous cell carcinoma, and liver hepatocellular carcinoma. Thus, serpin H1 may function as a potential prognostic biomarker for certain cancers (2).

Database Links

UniProt ID: P50454

Entrez-Gene Id: 871

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For Research Use Only. Not For Use In Diagnostic Procedures.

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