Render Target: STATIC
Render Timestamp: 2024-11-21T13:25:08.373Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:26:19.937
Product last modified at: 2024-10-11T22:45:07.460Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

HTATIP2/TIP30 Antibody #14614

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 28, 32
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HTATIP2/TIP30 Antibody recognizes endogenous levels of total HTATIP2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His231 of human HTATIP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The HIV-1 Tat interactive protein 2 (HTATIP2, TIP30, CC3) is an oxidoreductase that was originally identified as a metastatic tumor suppressor and Tat-mediated proapoptotic gene transcription cofactor (1,2). HTATIP2 protein contains a short-chain dehydrogenase (SDR) domain and a NADPH binding motif important for HTATIP2 interaction with importins and inhibition of nucleocytoplasmic transport (3,4). Research studies demonstrate that induced overexpression of HTATIP2 predisposes cells to apoptosis by inhibiting the nuclear transport of important signaling proteins (e.g. p53, activated notch1) and several key targets of the DNA repair process (5-7). HTATIP2 is part of a protein complex, with Rab5a, endophilin B1, and ACSL4, that may regulate EGFR receptor endosomal trafficking, degradation, and cytoplasmic/nuclear signaling (8,9). Silencing of HTATIP2 promotes tumor cell survival under low glucose conditions by inducing increased expression of mitochondrial respiratory proteins and glucose metabolic enzymes (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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