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Primary Antibodies /
Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free)

Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) #92511

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Mouse IgG2a kappa

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

Endotoxin

Less than or equal to 0.01 EU/μg, as determined by the LaL assay.

Formulation

Supplied in 10 mM NaH2PO4, 150 mM NaCl, pH7.2, Low Endotoxin, Azide-free.

Storage

Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) recognizes endogenous levels of CD3ε protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced against human PBMC cells and purified via affinity chromatography.

Background

When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).

Database Links

UniProt ID: P07766

Entrez-Gene Id: 916

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For Research Use Only. Not For Use In Diagnostic Procedures.

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