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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ICOS (E5F7E) Rabbit mAb #67223

Filter:
  • WB
  • IP
  • IHC
  • IF
Western Blotting Image 1: ICOS (E5F7E) Rabbit mAb
Western blot analysis of extracts from various cells and tissues using ICOS (E5F7E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). CD4+ T cells were purified from mouse spleens or human blood and stimulated for 7 days using beads coated with CD3 and CD28 antibodies in the presence of Mouse Interleukin-2 (mIL-2) (20 ng/ml) or Human Interleukin-2 (hIL-2) #8907 (20 ng/ml).

To Purchase # 67223

Cat. # Size Qty. Price
67223T 20 µl
$162
67223S 100 µl
$357

Supporting Data

REACTIVITY M R
SENSITIVITY Endogenous
MW (kDa) 22-35
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunohistochemistry (Paraffin) 1:50 - 1:200
Immunofluorescence (Frozen) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #18670.

Protocol

Specificity / Sensitivity

ICOS (E5F7E) Rabbit mAb recognizes endogenous levels of total mouse ICOS protein.

Species Reactivity:

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val198 of mouse ICOS protein.

Background

ICOS (Inducible Co-Stimulator, CD278) is a member of the CD28 family that regulates T cell activity and immune responses (1). The ICOS protein contains an extracellular IgV-like domain, a transmembrane domain, and an intracellular domain with a YMFM motif (1-2). ICOS is primarily expressed on activated CD4+ and CD8+ T cells (1). Upon binding to its ligand, ICOS potentiates the T cell response to antigen through activation of the PI3K signaling pathway (3). In addition to enhancing T cell activation and proliferation, ICOS plays an important role in the regulation of T follicular helper cells (4). Research studies suggest that ICOS is a potential therapeutic target, and could serve as a prognostic biomarker for neoplastic therapy involving CTLA-4 blockade (5-7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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