Render Target: STATIC
Render Timestamp: 2024-11-21T13:20:37.009Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:54:21.187
Product last modified at: 2024-09-30T08:02:23.965Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

IFI16 (D8B5T) Rabbit mAb #14970

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 85, 95, 98
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #89555.

    Protocol

    Specificity / Sensitivity

    IFI16 (D8B5T) Rabbit mAb recognizes endogenous levels of total IFI16 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro399 of human IFI16 protein.

    Background

    IFI16 belongs to the PYHIN family, which consists of four members in humans (Aim2, IFIX, MNDA, and IFI16) and at least thirteen members in mice (1). The closest homolog of IFI16 in mice is p204. The PYHIN family is characterized by the presence of one or two HIN-200 domains that bind double stranded DNA and a PYRIN domain that mediates interactions among family members, as well as the inflammasome-forming ASC protein (1). IFI16 also has a nuclear localization signal, but has been observed to bind DNA in both the cytoplasm and nucleus (2,3). There are three isoforms of IFI16 produced by alternative splicing (4). IFI16 is constitutively expressed by some cells, such as lymphocytes, and highly inducible in many others by interferons (5). IFI16 has been shown to directly interact with p53 and to play a role in cellular senescence by modulating p53 activity (6). IFI16 was shown to directly sense intracellular viral DNA, leading to recruitment of STING and activation of IRF-3 and NF-κB (2). Investigators have observed increased levels of IFI16 and anti-IFI16 autoantibodies in patients with systemic lupus erythematosus (7-9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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