Render Target: STATIC
Render Timestamp: 2024-12-26T10:59:39.666Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:19:21.790
Product last modified at: 2024-12-17T20:15:09.608Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

IκBα Antibody #9242

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk B Dg Pg
    SENSITIVITY Endogenous
    MW (kDa) 39
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • B-Bovine 
    • Dg-Dog 
    • Pg-Pig 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IκBα Antibody detects endogenous levels of total IκBα protein. Under some experimental conditions it binds preferentially to IκBα that is not phosphorylated at Ser32/Ser36.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Bovine, Dog, Pig

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Arg29 of human IκBα. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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