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IKKα (3G12) Mouse mAb (BSA and Azide Free) #49706

Filter:
  • WB
  • IF
  • ELISA
Western blot analysis of extracts from various cell lines using IKKα (3G12) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.

To Purchase # 49706

Cat. # Size Qty. Price
49706SF 100 µg
$843

Product Specifications

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 85
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

This product is the carrier free version of product #11930. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #11930.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

IKKα (3G12) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total IKKα protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a carboxy-terminal fragment of human IKKα protein.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).

Pathways

Explore pathways related to this product.


For Research Use Only. Not for Use in Diagnostic Procedures.
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