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Render Timestamp: 2024-12-26T12:07:10.261Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:32:20.508
Product last modified at: 2024-12-09T12:30:32.370Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. IKKε (D61F9) XP® Rabbit mAb

IKKε (D61F9) XP® Rabbit mAb #3416

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  • IP
  • IF
  • F

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:100
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #96794.

    Protocol

    Specificity / Sensitivity

    IKKε (D61F9) XP® Rabbit mAb detects endogenous levels of total IKKε protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of mouse IKKε.

    Background

    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
    Recently, two homologs of IKKα and IKKβ have been described, called IKKε (also known as IKK-i) and TBK-1 (also known as T2K or NAK), and activation of either of these kinases results in NF-κB activation. IKKε contains the kinase domain in its amino terminus, which shares 30% identity to that of IKKα or IKKβ. IKKε is expressed mainly in immune cells, and may play a special role in the immune response (14-18).
    1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
    2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
    3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
    4. Brown, K. et al. (1995) Science 267, 1485-8.
    5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
    6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
    7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
    8. Zandi, E. et al. (1997) Cell 91, 243-52.
    9. Karin, M. (1999) Oncogene 18, 6867-74.
    10. DiDonato, J.A. et al. (1997) Nature 388, 548-54.
    11. Mercurio, F. et al. (1997) Science 278, 860-6.
    12. Johnson, L.N. et al. (1996) Cell 85, 149-58.
    13. Delhase, M. et al. (1999) Science 284, 309-13.
    14. Shimada, T. et al. (1999) Int Immunol 11, 1357-62.
    15. Peters, R.T. et al. (2000) Mol Cell 5, 513-22.
    16. Tojima, Y. et al. (2000) Nature 404, 778-82.
    17. Bonnard, M. et al. (2000) EMBO J 19, 4976-85.
    18. Peters, R.T. and Maniatis, T. (2001) Biochim Biophys Acta 1471, M57-62.

    Database Links

    UniProt ID: Q14164


    Entrez-Gene Id: 9641


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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