IL-17A (D1X7L) Rabbit mAb #13838
- WB
- IP
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17, 14 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #90428.
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
IL-17A is a cysteine-linked, homodimeric, pro-inflammatory cytokine produced by Th17 cells, a distinct CD4+ T cell lineage (9,10). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNFα, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (9,10). IL-17A is intimately involved in mucosal immunity against bacterial infections (9,11) and has a putative role in some autoimmune disorders (9,12). IL-17A effects appear to be exerted primarily through binding to one of the IL-17 receptor subunits, IL-17RA (13). IL-17 binding induces production of cytokines, chemokines, and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (11,12,14). Phosphorylation of some Jaks and Stats has been observed.
- Gaffen, S.L. (2009) Nat Rev Immunol 9, 556-67.
- Iwakura, Y. et al. (2011) Immunity 34, 149-62.
- Hu, Y. et al. (2011) Ann N Y Acad Sci 1217, 60-76.
- Fort, M.M. et al. (2001) Immunity 15, 985-95.
- Yamaguchi, Y. et al. (2007) J Immunol 179, 7128-36.
- Li, H. et al. (2000) Proc Natl Acad Sci U S A 97, 773-8.
- Chang, S.H. et al. (2006) J Biol Chem 281, 35603-7.
- Shalom-Barak, T. et al. (1998) J Biol Chem 273, 27467-73.
- Kolls, J.K. and Lindén, A. (2004) Immunity 21, 467-76.
- Liang, S.C. et al. (2006) J Exp Med 203, 2271-9.
- Dubin, P.J. and Kolls, J.K. (2008) Immunol Rev 226, 160-71.
- Zrioual, S. et al. (2009) J Immunol 182, 3112-20.
- Wright, J.F. et al. (2008) J Immunol 181, 2799-805.
- Rahman, M.S. et al. (2006) J Immunol 177, 4064-71.
Limited Uses
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