Render Target: STATIC
Render Timestamp: 2024-11-15T10:37:03.787Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-04-05 20:46:14.999
Product last modified at: 2024-11-11T14:00:16.811Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

IL-1α Antibody #84618

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IL-1α Antibody recognizes endogenous levels of total IL-1α protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IL-1α protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Interleukin 1 alpha (IL-1α) belongs to the IL-1 family of cytokines with 11 members including IL-1β. IL-1α is expressed in many cell types of both hematopoietic and non-hematopoietic origins under steady state, and its expression can be increased in response to appropriate stimuli (1,2). Like IL-1β, IL-1α is also synthesized as a precursor (pro-IL-1α) and can be cleaved into smaller mature forms. However, both pro-IL-1α and the cleaved form of IL-1α are biologically active and can activate the signaling pathway through the membrane receptor IL-1R1. IL-1α is active both as a secreted form and as a membrane-bound form. Due to such characteristics, passive leakage of IL-1α from dying cells can activate inflammation, leading some researchers to consider IL-1α as a key “alarmin in the cell” that alerts the host to damage or injury (3,4). In addition, IL-1α can also enter the nucleus to modulate transcription (5,6).
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