Render Target: STATIC
Render Timestamp: 2024-12-23T12:03:15.289Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:15:36.070
Product last modified at: 2024-09-13T07:01:13.612Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

IL-1β Antibody #2022

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Inquiry Info. # 2022

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 17, 31
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IL-1 beta Antibody detects endogenous levels of both the 31 kDa precursor and 17 kDa mature form of IL-1 beta.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of the 17 kDa mature form of human IL-1 beta. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.
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