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IL-2Rα Antibody #5370

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Filter:
  • WB
Western Blotting Image 1: IL-2Rα Antibody
Western blot analysis of extracts from SR, KARPAS-299, and MOLT-4 cells using IL-2Rα Antibody.
This product is discontinued

Inquiry Info. # 5370

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Product Specifications

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 55
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

IL-2Rα Antibody detects endogenous levels of total human IL-2Rα protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Ala34 of human IL-2Rα protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).

Alternate Names

IL-2-Rα; IL-2R-α; IL2-Rα

For Research Use Only. Not for Use in Diagnostic Procedures.
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