IL-6Rα/CD126 (E7H4J) Rabbit mAb #39837

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)	40, 78
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

IL-6Rα/CD126 (E7H4J) Rabbit mAb recognizes endogenous levels of total IL-6Rα/CD126 protein. This antibody cross-reacts with an unidentified protein of 20 kDa in some cell extracts.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse IL-6Rα/CD126 protein.

Background

The IL-6 receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130 (1). Binding of IL-6 to IL-6Rα results in receptor dimerization with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17 (1,2). In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA (1,3). Soluble IL-6Rα binds to IL-6 and can stimulate signaling via membrane bound gp130 in a process known as “trans-signaling” (1). It is through trans-signaling that IL-6 stimulates cells that do not express membrane bound IL-6Rα (1).

Database Links

UniProt ID: P22272

Entrez-Gene Id: 16194

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For Research Use Only. Not For Use In Diagnostic Procedures.

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