Render Target: STATIC
Render Timestamp:
4/5/2025, 12:48:55 AM EDT
4/5/2025, 4:48:55 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:11:21.295
Product last modified at: 2025-03-24T12:15:09.496Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

IL1RAP (E2G6A) Rabbit mAb #73070

Filter:
  • WB
  • IP
Western Blotting Image 1: IL1RAP (E2G6A) Rabbit mAb
Western blot analysis of extracts from various cell lines using IL1RAP (E2G6A) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, IL1RAP protein expression is not detected in SU-DHL-4 cells. KARPAS-299 cell line source: Dr. Abraham Karpas at the University of Cambridge.

To Purchase # 73070

Cat. # Size Qty. Price
73070T 20 µl
$153
73070S 100 µl
$339

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 78
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

IL1RAP (E2G6A) Rabbit mAb recognizes endogenous levels of total IL1RAP protein.

Species Reactivity:

Human

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Dog, Horse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IL1RAP protein.

Background

Interleukin-1 receptor accessory protein (IL1RAP/IL1R3) is a type-I transmembrane glycoprotein and component of the IL-1 and IL-33 receptor signaling complexes (1,2). Research studies have demonstrated that IL1RAP expression is required for IL-1-mediated signaling through its ability to recruit IRAK to the IL-1R complex (3-7). In the context of human disease, research studies have shown that IL1RAP is overexpressed on the surface of AML stem cells and associates with c-KIT and FLT3 to mediate pro-proliferative and pro-survival signaling events downstream of these oncogenic receptor tyrosine kinases (8,9). As such, IL1RAP has emerged as a novel immuno-therapeutic target for AML and other malignancies of the myeloid compartment (10,11).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.