Render Target: STATIC
Render Timestamp: 2024-12-26T11:19:31.826Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:58.953
Product last modified at: 2024-12-17T18:53:42.534Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ILF3 (E5H6F) Rabbit mAb #39249

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 85, 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:1000 - 1:4000
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ILF3 (E5H6F) Rabbit mAb recognizes endogenous levels of total ILF3 protein. Species reactivity for IHC-P is human only.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val353 of human ILF3 protein.

    Background

    ILF2 and ILF3, also known as NF45 and NF90 respectively, form a double-stranded RNA binding heterodimer that serves many different biological functions. ILF2 and ILF3 were originally discovered as proteins that can bind and regulate the antigen receptor response element of the IL2 promoter to contribute to T cell activation (1,2). ILF2 and ILF3 play important roles in DNA damage response and non-homologous end joining (NHEJ) by interacting with DNA-PK (3,4). The heterodimer can also bind and negatively regulate microRNAs (5,6). Biogenesis of the 60S ribosomal subunit has been shown to be dependent on the double-stranded RNA-binding domains of ILF3 (7). Additionally, ILF2 and ILF3 can mediate rDNA transcription (8). ILF2 and ILF3 also play a role in the life cycle of many different viruses, including dengue and hepatitis C (9-11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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