Render Target: STATIC
Render Timestamp: 2024-11-22T11:44:35.569Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 02:00:01.181
Product last modified at: 2024-09-30T08:01:16.096Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ING5 (F7J4V) Rabbit mAb #80399

Filter:
  • WB
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Chromatin IP 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ING5 (F7J4V) Rabbit mAb recognizes endogenous levels of total ING5 protein. This antibody does not cross-react with ING4 protein. This antibody detects bands of unknown origin between 80 and 160 kDa.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro223 of human ING5 protein.

    Background

    Inhibitor of growth 5 (ING5) is a member of an evolutionarily conserved family of tumor suppressor proteins and transcription regulators, which contain a conserved PHD domain (1,2). This protein family has been implicated in inhibition of cellular growth and is differentially expressed in cancer (3). ING5 associates with complexes containing HBO1 or H3-specific MOZ histone acetyltransferases (HATs) and is critical to histone acetylation associated with DNA replication during S-phase (4). ING5 associates with the TIP60 acetyltransferase to acetylate Lys120 of p53, promoting apoptosis (5). ING5 is phosphorylated on Thr152 by CDK2 in a cell cycle-dependent manner, and can also control apoptosis in a p53-independent manner (6). ING5 has been shown to be a positive prognostic marker in various cancer types (7-9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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