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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Innate Immunity Activation Antibody Sampler Kit

Innate Immunity Activation Antibody Sampler Kit #52239

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Innate Immunity Activation Antibody Sampler Kit provides an economical means of detecting the activation of multiple signaling pathways involved in innate immunity using phospho-specific, cleavage-specific, and control antibodies. The kit contains enough primary antibodies to perform at least two western blot experiments.

    Specificity / Sensitivity

    Each antibody in the Innate Immunity Activation Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-STING (Ser366) (D7C3S) Rabbit mAb detects STING only when phosphorylated at Ser366. Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb detects IRF-3 only when phosphorylated at Ser396. Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb detects IRAK4 only when phosphorylated at both Thr345 and Ser346 and does not react with IRAK4 when phosphorylated at only one of these sites. Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb detects IRF-7 only when phosphorylated at Ser477. Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb may cross-react with proteins of unknown origin between 100 and 150 kDa. Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb detects mature IL-1β only when cleaved at Asp116.

    Source / Purification

    Monoclonal and polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro226 of STING, Lys41 of IRAK4, Pro115 of IRF-7, Asp116 of IL-1β, or recombinant human IRF-3 protein. Phospho-specific antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser366 of STING, Ser396 of IRF-3, Thr345/Ser346 of IRAK4, or Ser477 of IRF-7. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The innate immune system responds rapidly to pathogens by detecting conserved pathogen-associated molecular patterns (PAMPs) and damage/danger-associated molecular patterns (DAMPs) through pattern recognition receptors (PRRs). There are several families of PRRs. Toll-like receptors (TLRs) are transmembrane PRRs and signal through recruitment of adaptor proteins, including MyD88, which leads to recruitment and phosphorylation of IRAK1 and IRAK4, followed by activation of NF-κB and MAP kinases (1-3). Some TLRs also activate IRFs, which upregulate the type I interferon response. Activation of TLR3 and TLR4 results in phosphorylation and activation of IRF-3, while TLR7, TLR8, and TLR9 lead to activation of IRF-7 (2, 3). STING is a multi-pass ER transmembrane protein that is activated in response to intracellular DNA downstream of DNA-sensing cytoplasmic PRRs, such as DDX41, or by binding the second messenger cyclic-GMP-AMP (cGAMP) produced by cGAS (4-6). Following activation, STING translocates with TBK1 to perinuclear endosomes, leading to phosphorylation and activation of IRF-3 and NF-κB (7, 8). Following activation and translocation, STING gets phosphorylated by ULK1, resulting in STING inactivation and degradation (9). Inflammasomes are cytoplasmic multimeric protein complexes that assemble in response to PAMPs or DAMPs detected by AIM2 or members of the nod-like receptor (NLR) family, such as NLRP3 (10). Inflammasomes activate Caspase-1, which cleaves the IL-1β and IL-18 precursor proteins into the mature forms (10).

    Database Links

    UniProt ID: P01584 , Q92985 , Q86WV6 , Q14653 , Q9NWZ3


    Entrez-Gene Id: 3553 , 3665 , 340061 , 3661 , 51135


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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