Render Target: STATIC
Render Timestamp: 2024-11-12T10:20:22.412Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-04-05 20:44:27.425
Product last modified at: 2024-05-30T07:14:38.672Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

iNOS Antibody #39898

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Inquiry Info. # 39898

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 130
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    iNOS Antibody recognizes endogenous levels of total iNOS protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human iNOS protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body, including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

    NO catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi, and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins, such as LPS and proinflammatory cytokines, in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1, and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase, and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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