Integrin α4 (D2E1) XP® Rabbit mAb (BSA and Azide Free) #29873
- WB
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70, 140, 150, |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #8440
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
A pair of important α4 integrins, α4β1 and α4β7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that α4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin α4 blocks binding to paxillin and promotes leading edge migration (7,8).
On SDS-PAGE, integrin α4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (9). Integrin α4 has a cleavage site at Arg558, which results in a small portion of the protein as either an 80 kDa N-terminal or 70 kDa C-terminal fragment (10).
- Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
- Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
- Plow, E.F. et al. (2000) J Biol Chem 275, 21785-8.
- Bonder, C.S. et al. (2005) Immunity 23, 153-63.
- Arroyo, A.G. et al. (1999) Immunity 11, 555-66.
- Yang, J.T. et al. (1995) Development 121, 549-60.
- Nishiya, N. et al. (2005) Nat Cell Biol 7, 343-52.
- Alon, R. et al. (2005) J Cell Biol 171, 1073-84.
- Teixidó, J. et al. (1992) J Biol Chem 267, 1786-91.
- Pujades, C. et al. (1996) Biochem J 313 ( Pt 3), 899-908.
Limited Uses
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