Integrin β6 (E4M9P) XP® Rabbit mAb #95153
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 105 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 - 1:12800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #52976.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The avβ6 subunit was identified in epithelial cells and interacts with the fibronectin receptor integrin α5 (3,4). It is expressed during development, but expression becomes repressed in adulthood (5). It is involved in wound healing, fibrosis, pulmonary fibrosis in particular, and tumorigenesis (6-8). When the dimer is unregulated in the membrane in response to injury, the integrin β6 subunit binds directly to an RGD motif present in fibronectin and TGF-β, both present in the extracellular matrix. Integrin β6 cleaves and binds the LAP fragment of latent TGF-β, thereby releasing active TGF-β into the ECM to activate myofibroblasts which consequently mediate ECM remodeling (6-8).
- Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
- Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
- Breuss, J.M. et al. (1995) J Cell Sci 108 ( Pt 6), 2241-51.
- Sheppard, D. et al. (1990) J Biol Chem 265, 11502-7.
- Busk, M. et al. (1992) J Biol Chem 267, 5790-6.
- Haapasalmi, K. et al. (1996) J Invest Dermatol 106, 42-8.
- Munger, J.S. et al. (1999) Cell 96, 319-28.
- Bandyopadhyay, A. and Raghavan, S. (2009) Curr Drug Targets 10, 645-52.
Limited Uses
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