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Render Timestamp: 2024-12-27T12:12:14.888Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:19.790
Product last modified at: 2024-09-30T08:01:03.291Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Intersectin-2 (E5J9F) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Intersectin-2 (E5J9F) Rabbit mAb #43069

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 200, 140
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Intersectin-2 (E5J9F) Rabbit mAb recognizes endogenous levels of total intersectin-2 protein. This antibody reacts with intersectin-2L and intersectin-2S protein isoforms but does not cross-react with the corresponding isoforms of intersectin-1 protein. This antibody cross-reacts with proteins of unknown identity between 80 kDa and 120 kDa in some rat cell extracts.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys335 of human intersectin-2 protein.

    Background

    Intersectin-2 is a ubiquitously expressed, modular adaptor protein that participates in clathrin-mediated endocytosis through interaction with multiple proteins involved in trafficking of proteins through the endocytic pathway. Like its paralog, intersectin-1, there are two major isoforms of mammalian intersectin-2. Intersectin-2L and intersectin-2S are generated through alternative splicing of mRNA transcripts. The structure of both isoforms is characterized by two N-terminal EH domains, a central coiled-coil domain, followed by five tandem SH3 domains. The longer isoform, intersection-2L, contains an extended C-terminus with a Rho-GEF domain that controls the activity of the Cdc42 GTPase, a PH domain, and a calcium binding C2 domain (1-3). Research studies have shown that intersectin-2 plays a role in T cell receptor (TCR) signaling by promoting endocytosis of the TCR following its activation (4).

    Database Links

    UniProt ID: Q9NZM3


    Entrez-Gene Id: 50618


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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