Render Target: STATIC
Render Timestamp: 2024-12-20T12:02:30.269Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-05 20:24:35.596
Product last modified at: 2024-10-08T12:00:13.268Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

INTS9 Antibody #13945

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 75
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    INTS9 Antibody recognizes endogenous levels of total INTS9 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Hamster, Bovine, Dog, Pig

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His601 of human INTS9 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The integrator complex is an evolutionarily conserved complex that is composed of at least 12 subunits in humans. It is thought to be a multifunctional complex with roles in orchestrating snRNA 3' end processing with transcription termination, DNA double-stranded break repair, hematopoietic development, and cell cycle progression (1-6). The integrator subunits (INTS) 9 and 11 are thought to be the catalytic subunits of the complex and are essential for the function of the complex (6,7). Research studies indicate that the integrator complex is recruited to snRNA genes through its interaction with the carboxy-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II (8). Phosphorylation of the Rpb1 CTD heptapeptide repeat residues Ser2 and Ser7 is required for efficient binding of integrator subunit proteins (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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