IRF-3 Antibody #4962
Inquiry Info. # 4962
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Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 52 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
IRF3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF3, leading to its translocation to the nucleus, where it activates promoters containing IRF3-binding sites. Phosphorylation of IRF3 occurs at a cluster of serine and threonine residues in the carboxy-terminus between residues 385 and 405. This leads to an association with the p300/CBP coactivator and stimulation of DNA binding and transcriptional activity (5). During infection, IRF3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKe and TBK1 (6,7).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Hiscott, J. et al. (1999) J Interferon Cytokine Res 19, 1-13.
- Kim, T.Y. et al. (2003) J Biol Chem 278, 15272-8.
- Yoneyama, M. et al. (2002) J Interferon Cytokine Res 22, 73-6.
- Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
- Kopp, E. and Medzhitov, R. (2003) Curr Opin Immunol 15, 396-401.
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