IRF-6 (E5C5D) Rabbit mAb #60615
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 58, 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
While IRF family members generally function in innate immune responses, IRF-6 has not been associated in that role. Original studies of IRF-6 found that mutation of the IRF-6 gene caused Van der Woude Syndrome, an autosomal dominant disorder resulting in mouth abnormalities including cleft lip and palate (3). IRF-6 knockouts show a hyperproliferative epidermis that fails to undergo keratinocyte differentiation (4). IRF-6 has also been found to interact with the mammary tumor suppressor maspin, and like maspin is expressed in normal mammary epithelial but reduced or absent in breast carcinoma (5). Cellular proliferation may promote IRF-6 phosphorylation leading to its proteasomal dependent degradation (6).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Kondo, S. et al. (2002) Nat Genet 32, 285-9.
- Richardson, R.J. et al. (2006) Nat Genet 38, 1329-34.
- Bailey, C.M. et al. (2005) J Biol Chem 280, 34210-7.
- Bailey, C.M. et al. (2008) Mol Cell Biol 28, 2235-43.
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