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Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:14:40.706
Product last modified at: 2025-03-18T18:00:09.623Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

IRG1 Antibody #17805

Filter:
  • WB
Western Blotting Image 1: IRG1 Antibody
Western blot analysis of extracts from RAW 264.7 cells, untreated (-) or treated with LPS #14011 (5 μg/ml, 6 hr; +), using IRG1 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 17805

Cat. # Size Qty. Price
17805S 100 µl
$306

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 50
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

IRG1 Antibody recognizes endogenous levels of total IRG1 protein.

Species Reactivity:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse IRG1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

IRG1 (Immune-responsive gene 1) is one of the most up-regulated genes in macrophages under proinflammatory conditions (1). It is also highly expressed in the pregnant uterus during implantation (2,3). IRG1 is a cis-aconitate decarboxylase that produces itaconic acid by decarboxylating cis-aconitic acid, an intermediate of the tricarboxylic acid cycle (4). Itaconic acid is an endogenous inhibitor of succinate dehydrogenase, linking macrophage metabolic rewiring and regulation of inflammation (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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