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Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:31.630
Product last modified at: 2024-09-30T08:02:46.396Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. ITM2B/Bri2 (E6O3Y) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ITM2B/Bri2 (E6O3Y) Rabbit mAb #10644

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 42, 84
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ITM2B/Bri2 (E6O3Y) Rabbit mAb recognizes endogenous levels of total human ITM2B/Bri2 protein. Bands at 84 kDa and 21 kDa are occasionally detected, likely representing oligomers of ITM2B/Bri2 and cleavage products, respectively. This antibody does not cross-react with rodent orthologs of ITM2B/Bri2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala105 of human ITM2B/Bri2 protein.

    Background

    Integral membrane protein 2b (ITM2B), also known as Bri2, is a type II membrane protein. ITM2B is expressed as a precursor immature form and is processed by furin proteases to produce the mature ITM2B/Bri2 protein and a soluble C-terminal fragment (1,2). The membrane-bound ITM2B/Bri2 protein can be further processed by ADAM10 and intramembrane proteases (1). Several studies have implicated the ITM2B gene with familial forms of dementia and neurodegenerative diseases, including Alzheimer’s disease (AD). Mutations in the human ITM2B gene are linked to several familial British and Danish dementia disorders (3,4). ITM2B gene mutations cause abnormal processing of the ITM2B/Bri2 protein, suggesting that the products of ITM2B/Bri2 protein cleavage might contribute directly to disease etiology (4). Interestingly, cleavage of disease-linked mutant ITM2B-encoded proteins generates peptides (ABri and ADan) that are more prone to deposit as amyloid fibrils, a pathological hallmark of many neurodegenerative diseases (2,4). Additionally, ITM2B/Bri2 interacts with Aβ-precursor protein (APP), a gene/protein linked to AD, and may alter APP processing and fibril formation (5-7). ITM2B/Bri2 is one of a family of proteins containing a BRICHOS domain, identified by their ability to inhibit Aβ fibril formation (8). The specific function of ITM2B/Bri2 is unclear, but it may contribute to normal synaptic function via an unknown mechanism (9).

    Database Links

    UniProt ID: Q9Y287


    Entrez-Gene Id: 9445


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