Render Target: STATIC
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XML generation date: 2024-09-20 06:21:32.859
Product last modified at: 2025-01-06T18:15:08.309Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

Jak/Stat Pathway Inhibitors Antibody Sampler Kit #8343

Jak/Stat Pathway Inhibitors Antibody Sampler Kit: Image 1
Western blot analysis of extracts from K562, A204 and PC12 cells using SOCS2 Antibody.

To Purchase # 8343

Cat. # Size Qty. Price Ships
8343T 1 Kit
6 x 20 microliters
$558
  • Product Includes
  • Related Products
Product IncludesQuantityApplicationsReactivityMW(kDa)Isotype
SOCS1 (E3Q4M) Rabbit mAb #5531320 µlWB, IPH M R Mk23Rabbit IgG
SOCS2 Antibody #277920 µlWB, IPH M R22Rabbit 
SOCS3 (D6E1T) Rabbit mAb #5211320 µlWBH M28Rabbit IgG
PIAS1 (D33A7) XP® Rabbit mAb #355020 µlWB, IF, FH M R Mk76Rabbit IgG
PIAS3 (D5F9) XP® Rabbit mAb #904220 µlWB, IP, IFH65-75Rabbit IgG
PIAS4 (D2F12) Rabbit mAb #439220 µlWBH R Mk75Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074100 µlWBRabGoat 

Product Information

Product Description

The Jak/Stat Pathway Inhibitors Antibody Sampler Kit provides an economical means to examine several inhibitors of Jak/Stat signaling, including PIAS1, PIAS3, PIAS4, SOCS1, SOCS2, and SOCS3. The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human SOCS2 protein, or recombinant protein specific to human SOCS3 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser550 of human PIAS1 protein, Pro590 of human PIAS3 protein, Lys59 of human PIAS4 protein, or near the carboxy terminus of human SOCS1 protein.

Background

Jak (Janus Kinase) and Stat (signal transducer and activator of transcription) proteins are utilized by receptors for a wide varity of ligands including cytokines, hormones, growth factors, and neurotransmitters (1). Jaks and Stats play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses, and stem cell differentiation (2-5). Therefore, regulation of Jak/Stat signaling is crucial to prevent aberrant signaling which can lead to disease progression. Two methods for regulating Jak/Stat signaling involve SOCS and PIAS proteins (6,7).The SOCS (suppressor or cytokine signaling) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway and consist of 8 known members, including the originally identified protein CIS1 (cytokine-inducible SH2-containing protein) and SOCS1-SOCS7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses (8-10). SOCS proteins are involved in regulating over 30 cytokines, including interleukins, growth hormone (GH), interferons, leptin, and leukemia inhibitory factor (7). SOCS1, also known as JAB (Janus Kinase binding protein) and SSI-1 (Stat-induced Stat inhibitor-1), shares the most homology with SOCS3 and both are highly induced by cytokines (7,11). Both SOCS1 and SOCS3 directly inhibit Jak activity; SOCS1 inhibits Jak through an interaction involving a phospohotyrosine located in the kinase activation loop; SOCS3 inhibits Jak via its SH2 domain (12,13). In addition to inhibiting Jak/Stat signaling, the SOCS box of SOCS1 and SOCS3 can trigger ubiquitin-mediated degradation of proteins within and outside the Jak/Stat pathway (14,15). SOCS2 is also incduced upon cytokine stimulation and the activity of SOCS2 has been predominately linked to GH and insulin-like growth fac
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