Render Target: STATIC
Render Timestamp: 2024-11-22T12:06:53.282Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:21:32.859
Product last modified at: 2024-11-12T16:00:08.601Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Jak/Stat Pathway Inhibitors Antibody Sampler Kit

Jak/Stat Pathway Inhibitors Antibody Sampler Kit #8343

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Jak/Stat Pathway Inhibitors Antibody Sampler Kit provides an economical means to examine several inhibitors of Jak/Stat signaling, including PIAS1, PIAS3, PIAS4, SOCS1, SOCS2, and SOCS3. The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human SOCS2 protein, or recombinant protein specific to human SOCS3 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser550 of human PIAS1 protein, Pro590 of human PIAS3 protein, Lys59 of human PIAS4 protein, or near the carboxy terminus of human SOCS1 protein.

    Background

    Jak (Janus Kinase) and Stat (signal transducer and activator of transcription) proteins are utilized by receptors for a wide varity of ligands including cytokines, hormones, growth factors, and neurotransmitters (1). Jaks and Stats play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses, and stem cell differentiation (2-5). Therefore, regulation of Jak/Stat signaling is crucial to prevent aberrant signaling which can lead to disease progression. Two methods for regulating Jak/Stat signaling involve SOCS and PIAS proteins (6,7).The SOCS (suppressor or cytokine signaling) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway and consist of 8 known members, including the originally identified protein CIS1 (cytokine-inducible SH2-containing protein) and SOCS1-SOCS7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses (8-10). SOCS proteins are involved in regulating over 30 cytokines, including interleukins, growth hormone (GH), interferons, leptin, and leukemia inhibitory factor (7). SOCS1, also known as JAB (Janus Kinase binding protein) and SSI-1 (Stat-induced Stat inhibitor-1), shares the most homology with SOCS3 and both are highly induced by cytokines (7,11). Both SOCS1 and SOCS3 directly inhibit Jak activity; SOCS1 inhibits Jak through an interaction involving a phospohotyrosine located in the kinase activation loop; SOCS3 inhibits Jak via its SH2 domain (12,13). In addition to inhibiting Jak/Stat signaling, the SOCS box of SOCS1 and SOCS3 can trigger ubiquitin-mediated degradation of proteins within and outside the Jak/Stat pathway (14,15). SOCS2 is also incduced upon cytokine stimulation and the activity of SOCS2 has been predominately linked to GH and insulin-like growth fac
    1. Darnell, J.E. et al. (1994) Science 264, 1415-21.
    2. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
    3. Dentelli, P. et al. (1999) J Immunol 163, 2151-9.
    4. Cattaneo, E. et al. (1999) Trends Neurosci 22, 365-9.
    5. Su, L. et al. (1999) J Biol Chem 274, 31770-4.
    6. Shuai, K. (2006) Cell Res 16, 196-202.
    7. Croker, B.A. et al. (2008) Semin Cell Dev Biol 19, 414-22.
    8. Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
    9. Chen, X.P. et al. (2000) Immunity 13, 287-90.
    10. Hilton, D.J. et al. (1998) Proc Natl Acad Sci U S A 95, 114-9.
    11. Starr, R. et al. (1997) Nature 387, 917-21.
    12. Yasukawa, H. et al. (1999) EMBO J 18, 1309-20.
    13. Sasaki, A. et al. (1999) Genes Cells 4, 339-51.
    14. Kamizono, S. et al. (2001) J Biol Chem 276, 12530-8.
    15. Rui, L. et al. (2002) J Biol Chem 277, 42394-8.
    16. Dey, B.R. et al. (1998) J Biol Chem 273, 24095-101.
    17. Liu, B. et al. (1998) Proc Natl Acad Sci U S A 95, 10626-31.
    18. Chung, C.D. et al. (1997) Science 278, 1803-5.
    19. Arora, T. et al. (2003) J Biol Chem 278, 21327-30.
    20. Nishida, T. et al. (2007) Biochem J 405, 481-8.
    21. Zhou, S. et al. (2008) J Biol Chem 283, 20137-48.

    Database Links

    UniProt ID: O15524 , Q8N2W9 , O14508 , Q9Y6X2 , O14543 , O75925


    Entrez-Gene Id: 8651 , 51588 , 8835 , 10401 , 9021 , 8554


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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