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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Jak2 (E4Y4D) Mouse mAb #74987

Filter:
  • WB
  • IP
Western Blotting Image 1: Jak2 (E4Y4D) Mouse mAb
Western blot analysis of extracts from various cell lines using Jak2 (E4Y4D) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 74987

Cat. # Size Qty. Price Ships
74987T 20 µl
$152
74987S 100 µl
$336

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 125
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Jak2 (E4Y4D) Mouse mAb recognizes endogenous levels of total Jak2 protein. This antibody does not cross-react with human Jak1, Jak3, or Tyk2 proteins.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Jak2 protein.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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